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Initial pre-induction stages utilize compounds like nicotinamide, -mercaptoethanol, and retinoic acid, followed by exposure to high-glucose Dulbeccos Modified Eagle Medium (DMEM) supplemented with growth factors such as activin A, hepatocyte growth factor (HGF), or epidermal growth factor (EGF)

These can signal deeper impacts such as electrolyte imbalance , low-grade inflammation , or hydration issues (Nurseslabs, USC Leonard Davis School of Gerontology, San Francisco Chronicle)

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