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At the end point, cells were collected by trypsinization (the culture medium and PBS washes were collected and re-added to each sample) and washed once with assay buffer (10 mM HEPES, 140 mM NaCl and 3.3 mM CaCl 2 , pH 7.4)

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Purity: 99.72% CAS No.: 111470-99-6 Formula: C 26 H 31 ClN 2 O 8 S Molecular Weight:567.05 Storage: 4C, sealed storage, away from moisture * In solvent : -80C, 6 months

This ensures proper tracking and prevents any mix-ups

Frontiers in Endocrinology, 11, 178