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Briefly, NIC cells seeded on Seahorse 96-well plates were cultured in substrate-limited DMEM ([-] D-glucose, [-] Sodium Pyruvate (Wisent, 319-062-CL)) with 0.5 mM D-glucose (BioShop #GLU501), 1 mM GlutaMAX (Sigma Aldrich, #G7021), 0.5 mM Carnitine (Sigma Aldrich, #C0283) and 1% FBS (Wisent, 080-150)

GLP-1

Dimitris Papamargaritis has acted as a speaker for Novo Nordisk, Eli Lilly and Boehringer Ingelheim and Johnson and Johnson and has received grants from Novo Nordisk, Novo Nordisk UK Research Foundation, Academy of Medical Sciences/Diabetes UK, Health Education East Midlands and the NIHR

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These estimates were based on patients with available follow-up weight measurements, representing a subset of the full study population

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