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Chromatin immunoprecipitation (ChIP) assay The ChIP assay was performed as previously described 41 , with slight modifications
Treatment of J744A.1 macrophage cell line with 500 M and pathophysiological concentrations (800 M) of UA induces antioxidant cell response proved by the indicative elevation of GCL, GPx1, GR, and GS transcription
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Therefore, to determine the specificity of the complete loss of H3K27me3 expression in the context of the differential diagnosis of malignant peripheral nerve sheath tumor from melanoma (its major potential mimic), we performed H3K27me3 immunohistochemistry in a pathologically and genetically well-characterized cohort of primary (neurofibromatosis type 1 (NF1), radiation-associated and sporadic context) malignant peripheral nerve sheath tumors ( n =122) and in a cohort or primary (desmoplastic) and metastatic melanomas ( n =265)
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