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Semaglutide was approved for chronic weight management in non-diabetic patients (as Wegovy) based on STEP trial data showing sustained weight loss across BMI categories and metabolic profiles

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Fluorescence/luminescence/absorbance intensities at appropriate wavelengths were quantified in Synergy H1 microplate reader (version 2.09.1)

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Finally, the pathway analysis was performed using ShinyGO v0.80 83 and Reactome 84 to investigate the enriched pathways associated with the significant proteins identified by the Random Forest method and Volcano plots