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Nitrous Oxide Use and Anemia Because nitrous oxide use can affect how your body absorbs vitamin B12, it can also lead to a condition called pernicious anemia

However, its more commonly sold as an intramuscular injection

There is no FDA-approved human trial

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The Complete Guide to B12 Shots Rebekah Jun 4, 2025 12 min read Updated: Nov 3, 2025 B12 shots are injectable forms of vitamin B12 (cobalamin) administered directly into muscle tissue, typically in the arm or thigh

Monitor for allergic reactions and discontinue the supplement if any signs of rash or breathing difficulties occur