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Together these modifications reduce IGFBP binding affinity by approximately 1,000-fold compared to native IGF-1 while preserving IGF-1 receptor (IGF1R) binding

Statistical Analysis To examine the primary impacts of the canola genotype and stress conditions, as well as their interactions, all gathered data and parameters were put through an analysis of variance (ANOVA) using SPSS statistics 22 and Statistix 10 (SPSS, Inc., Chicago, IL)

As expected, there were also decreased levels of TNF- and ICAM-1 expression through depression of NF-kB activation [47]

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