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Isopropyl -d-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM and the culture was further incubated at 18 C overnight

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Sterile water lacks preservatives and is single-use only (high contamination risk if reused)

Administration site: Subcutaneous injection as close to the affected spinal segment as practical

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When the supply of amino acids is reduced, it affects the renewal of cells, which causes, in particular, the thinning of the dermis