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approximately 99% of serum IGF-1 is protein-bound at any moment [1]

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This brain-derived neurotrophic function also prevents neurological disorders from forming

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Chen Y, Zhang R, Wang L, Correa AM, Pataer A, Xu Y, Zhang X, Ren C, Wu S, Meng QH, Fujimoto J, Jensen VB, Antonoff MB, Hofstetter WL, Mehran RJ, Pisimisis G, Rice DC, Sepesi B, Vaporciyan AA, Walsh GL, Swisher SG , Roth JA, Heymach JV, Fang B

Cells were spun at 4C for 20 min to pellet, and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH = 8)