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Moreover, N-myristoylation of ARF1 inhibits innate antiviral immune responses against herpes simplex virus1 infection by suppressing STING-induced IFN responses in macrophages while promoting STING-triggered autophagy, suggesting a therapeutic strategy for modulating STING activation ( 168 )

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For absolute quantitation of PCR reaction of each sample, amplification was performed, in parallel, on a standard curve of circularized pIRES2 plasmid, properly quantified and, on 3 l purified end joining reaction sample using CGTGTACGGTGGGAGGTCCTA forward primer and GGTACCGTCGACTGCAGAAT reverse primer, which span the sites of enzymatic restriction

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