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Both RAW 264.7 cells and peritoneal macrophages were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum, 5% CO, and 95% air at 37C

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7,8 Alterations in DNA damage-response pathways have been further associated with immune deficiency, infertility, premature aging and the development of atherosclerosis

NNMT (nicotinamide N-methyltransferase) is an enzyme in adipose tissue that depletes NAD+ and promotes fat storage