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The cells were incubated overnight at 4 C with the mouse anti-nestin antibody (1:200, Merck KGaA, Darmstadt, Germany), mouse anti-Tubulin III (1:200 Merck KGaA) and rabbit anti-glial fibrillary acidic protein (1:300 Dako Agilent Technologies, Santa Clara, CA, USA)
Conclusions : Lower plasma concentrations of glutathione suggest lower intracellular concentrations of the tripeptide
Storage: Both reconstituted peptides should be refrigerated at 2-8 degrees Celsius and are generally stable for 4-6 weeks after reconstitution
PMID 25274113
Removing the blockage typically resolves the issue
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