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GST-WWP-1 was eluted with glutathione elution buffer (50 mM Tris pH 8.0, 10 mM reduced glutathione) and buffer-exchanged into storage buffer (50 mM Tris pH 7.5, 50 mM NaCl, 5% glycerol, 1 mM DTT) using a NAP-10 column

Rajagopalan, S., Andreeva, A., Rutherford, T