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Briefly, preadipocytes expressing WT-IR and L973F-IR were serum starved in high-glucose DMEM supplemented with 0.1% BSA for 3 hours, followed by stimulation with 100 nM insulin for 0, 30, and 120 minutes

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Research-grade 5-Amino-1MQ refers to laboratory-use material tested for purity, analytical consistency, and controlled research applications

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