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Following treatment of yeast with lithium acetate and polyethylene glycol solutions
coli for DNA transformation
and algae)
The recombinant methylase is isolated from an E
Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads
CpG Methylase (M. Sssl) Name:CpG Methylase (M. Sssl) Following treatment of yeast withCpG Methylase completely methylates all cytosines in double stranded, non methylated, and hemimethylated DNA in a 5' CpG 3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.
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